METHOD FOR QUANTITATIVE DETERMINATION OF TAURIN AND ALLANTOINE PRESENT TOGETHER BY HPLC

FIELD: pharmacology.SUBSTANCE: invention relates to a method for quantitative determination of taurin and allantoin in their joint presence in various medicinal preparations, dietary supplements, cosmetic and food products by HPLC. Method involves dissolution of a substance portion in the mobile phase (MP), solution separation on a chromatographic column, measurement of the optical density of the resulting solution and determination of the concentrations of the studied substances by calibration charts. 30 mcmol/l of sodium acetate solution pH 6.0 was used as the mobile phase for taurin, and dissolution was performed at the rate of 250 mg of the substance per 10 ml with adjustment to volume with a MP solution. A mixture of a solution of ammonium hydrogen phosphate with pH 7.78 and acetonitrile 9:1 was used as a mobile phase for allantoin, and dissolution was performed at the rate of 1000 mg of the substance per 10 ml with adjustment to volume with a MP solution. For taurin, before MP solution splitting on the chromatographic column, borate buffer solution with pH of 9.0 and 0.1% solution of 2,4-dinitrochlorobenzene in a solution of acetonitrile-water (2:1) at a ratio of 1:1:1 is added to it, the mixture is heated, cooled to a room temperature and 1 vol. p. of 10% acetic acid and about 13 vol. p. of water was added per 6 vol. p. of mixture. Detection was carried out at wavelengths of 360±2 nm and 218±2 nm for taurin and allantoin, respectively.EFFECT: method allows to identify and quantify taurin and allantoin in their joint presence in various medicinal preparations.9 dwg Изобретение относится к способу количественного определения методом ВЭЖХ таурина и аллантоина при их совместном присутствии в различных лекарственных препаратах, биологически активных добавках, косметической и пищевой продукции. Способ включает растворение навески исследуемого вещества в подвижной фазе (ПФ), разделение раствора на хроматографической колонке, измерение оптической плотности полученного раствора и определение концентрации исследуемых веществ по калибровочным графикам. В качестве подвижной фазы для таурина использовали 30 мкмоль/л раствора ацетата натрия рН 6.0, а растворение проводили из расчета 250 мг исследуемого вещества на 10 мл и доведение до объема раствором ПФ. В качестве подвижной фазы для аллантоина использовали смесь раствора гидрофосфат аммония с рН 7.78 и ацетонитрила 9:1, а растворение проводили из расчета 1000 мг исследуемого вещества на 10 мл и доведение до объ