METHOD FOR STIMULATION OF ELECTROVIBRARY NEURONAL CELLS

FIELD: medicine.SUBSTANCE: method involves placing the cell cultures on a microelectrode array cup made in the form of an array of metal microelectrodes formed on a substrate with perimeter contact areas connected via conductive paths to microelectrodes. The viability of cell cultures is maintained under conditionsof CO-incubator. Generate electrical signals using a stimulator, transfer the generated signals from the stimulator to cell cultures on MEM, record the spontaneous activity of cell cultures. New is that MEM is pre-installed in a connector placed in an incubator. Moreover, in the connector above the microelectrode matrix (MEM), a board with an aperture is installed, with a projection, with pressure spring contacts connected by conductive paths. Install in such a way that the bowl with a culture of cells protrudes through the hole of the board, and the pressure spring contacts of the board are located coaxially with the contact areas of the MEM with the possibility of interacting with them. The connector and MEM are connected to the stimulator by means of a loop of wired connections. Generation of electrical signals is carried out in the form of a sequence consisting of a series of 5 pulses with an inter-pulse interval of 20 ms and an interval between the 200 ms series. In this case, the transfer of generated bipolar stimuli from the stimulator to the cell cultures is carried out by means of a loop from the wire connections connected to the connector at the output of the stimulator from one side and to the external connector of the connector on the other hand through the pressure spring contacts along the conductor tracks of the board to the contact areas of the MEM and along the conductive paths of MEM through microelectrodes.EFFECT: possibility of continuous and prolonged stimulation of the culture of electroexcitable neuronal cells, the improvement of the physiological conditions necessary for its development, and the enhancement of the reliability of the experiment.8 cl, 5 dwg, 1 ex Изобретение относится к медицине и физиологии и касается стимуляции электровозбудимых нейрональных клеток, что может быть использовано в исследованиях клеток, выращиваемых на микроэлектродной матрице (МЭМ). Способ включает размещение культур клеток на чаше микроэлектродной матрицы, выполненной в виде массива из металлических микроэлектродов, сформированных на подложке, с контактными площадками по периметру, соединенными посредством токопроводящих дорожек с микроэлек