OPTICAL METHOD FOR ASSESSING FUNCTIONAL STATE OF PLANTS

FIELD: biology.SUBSTANCE: present invention relates to experimental biology, plant growing, agriculture and forestry. Method involves the measurement of optical parameters of chlorophyll-containing tissues. Light scattering of photosynthesizing plant tissue is recorded when flash exposing the blue spectrum (in the area of the first maximum of chlorophyll absorption 460-480 nm) to monochromatic optical radiation with the power density of 150-800 w/mfor 20-40 seconds. Then the monochromatic probe radiation of red spectrum (in the area of the second maximum of chlorophyll absorption 650-660 nm) is activated with the power density of 2,500-6,000 w/m. For the following 30-120 seconds the dynamics of light scattering of the same tissue area continue to be registered. Photosynthesis activity and photodegradation resistance is determined based on the value and sign of values α and β, calculated using the formulas:andwhere |α| is the speed of light scattering intensity during exposure to blue spectrum optical radiation; Iis average light scattering intensity in the first 1-3 seconds of exposure to blue spectrum optical radiation; |β| is the speed of light scattering intensity during exposure to red spectrum optical radiation; Iis average light scattering intensity in the first 1-3 seconds during exposure to red spectrum optical radiation; t is current time. Higher the value of the modules of these indicators at negative sign, the higher the photosynthetic activity and photodegradation resistance, and the higher the value of the modules of indicators α and β at positive sign, the weaker the photosynthetic activity and resistance to photodegradation.EFFECT: method reduces labour input needed to assess the functional state of plants and increase its efficiency by means of quantitative evaluation of the photosynthesis activity and resistance to photodegradation of chlorophyll-containing tissues during one measurement cycle.1 cl, 1 dwg, 1 ex, 1 tbl Изобретение относится к области экспериментальной биологии, растениеводству, сельскому и лесному хозяйствам. Способ включает измерение оптических параметров хлорофиллсодержащих тканей. При этом регистрируют динамику светорассеяния фотосинтезирующей растительной ткани в процессе засветки монохроматическим оптическим излучением синей области спектра (в зоне первого максимума поглощения хлорофилла 460-480 нм) плотностью мощности 150-800 Вт/мв течение 20-40 секунд. Затем активируют монохроматическое зондирующее излучение красной