METHOD OF PRODUCTION OF HYPERIMMUNE SERUM AGAINST ANAEROBIC ENTEROTOXAEMIA AND COLIBACILLOSIS DIARRHOEA IN CALVES

FIELD: veterinary medicine.SUBSTANCE: method comprises hyperimmunisation of bulls-producers with inactivated antigens C1. perfringens, comprising toxoids and somatic antigens of bacteria of serotypes A, C and D in the culture medium with the following ratio of components per 1 litre of antigen: toxoid and somatic antigen of strain No 28 (type A) in the culture medium with a concentration of 10· (9-10) m.c. in 1 cm, cm- 300.0-350.0; toxoid and somatic antigen of strain No 392 (type C) in the culture medium at a concentration of 10· (9-10) m.c. in 1 cm, cm- 300.0-350.0; toxoid and somatic antigen of strain No 213 (type D) in the culture medium at a concentration of 10· (9-10) m.c. in 1 cm, cm- 300.0-350.0; formalin, cm- 4.0-5.0. The additional hyperimmunisation of bulls-producers is carried out with inactivated antigens of E.coli, comprising somatic and adhesive antigens K99, A20 in saline, TL-, TS-toxoids of E.coli in the culture medium in the following ratio of components per 1 litre of antigen: somatic and adhesive antigen of strain KV-1 (K99) in saline with a concentration of 10· (14-15) bln.c. in 1 cm, cm- 300.0-350.0; somatic and adhesive antigen of strain PZ-3 (A20) in saline with a concentration of 10· (14-15) m.c. in 1 cm, cm- 300.0-350.0; formalin, cm- 4.0-5.0; thermostable and thermolabile toxoids of E. coli strains KV-1 and PZ-3 in the culture medium (DPR) 1:8-1:16, l - to 1. Hyperimmunisation of bulls-producers is carried out four times by subcutaneous injection in the neck region on one side of the antigen dose C1. perfringens and in the neck region on the other side - E.coli antigen dose, according to the following scheme: on the first day - 8 cm, after 14 days - 10 cm, after 28 days - 15 cm, and after 42 days - 20 cmfrom blood, then serum is separated and preserved.EFFECT: invention with the further intake enables to obtain the hyperimmune serum, which provides treatment and prevention of mixed infection - anaerobic enterotoxaemia and colibacillosis diarrhoea of calves.5 tbl, 7 ex Изобретение относится к области ветеринарии и предназначено для получения гипериммунной сыворотки против анаэробной энтеротоксемии и эшерихиозной диареи телят. Способ включает гипериммунизацию быков-продуцентов инактивированными антигенами C1. perfringens, содержащими анатоксины и соматические антигены бактерий серотипов А, С и Д в культуральной среде при следующем соотношении компонентов на 1 л антигена: анатоксин и соматический антиген штамма №28 (тип А) в культур