SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELLED PROBE FOR IDENTIFICATION OF DNA OF ADENOVIRUSES OF SEROTYPES 3, 4, 7, 14, 21 BY METHOD OF HYBRIDISATION-FLUORESCENCE POLYMERASE CHAIN REACTION

FIELD: chemistry.SUBSTANCE: invention relates to field of biotechnology and deals with set, which includes oligodeoxyribonucleotide primers and fluorescently labelled probe for identification of DNA of adenovirus of serotypes 3, 4, 7, 14, 21 by method of hybridization-fluorescence polymerase chain r...

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Hauptverfasser:	TERNOVOJ VLADIMIR ALEKSANDROVICH, SERGEEV ALEKSANDR NIKOLAEVICH, SERGEEVA ELENA IGOREVNA, AGAFONOV ALEKSANDR PETROVICH
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Zusammenfassung:	FIELD: chemistry.SUBSTANCE: invention relates to field of biotechnology and deals with set, which includes oligodeoxyribonucleotide primers and fluorescently labelled probe for identification of DNA of adenovirus of serotypes 3, 4, 7, 14, 21 by method of hybridization-fluorescence polymerase chain reaction in real time mode. Claimed primers and probe have the following structure: external primer 5'?3' 5'-AATGTARTTGGGTCTGTTRGGCAT-3' internal primers 5'?3' 5'-CCCWTCGATGMTGCCCC-3' 5'-TCMACGGGYACRAAGCGCA-3' probe 5'?3' ROX-CCTGTCCGGCGATGTGCAT-BHQ2.EFFECT: invention possesses higher homology to currently circulating adenoviruses and makes it possible to identify DNA of adenoviruses of serotypes 3, 4, 7, 14, 21.3 dwg, 1 tbl Изобретение относится к области биотехнологии и касается набора, включающего олигодезоксирибонуклеотидные праймеры и флуоресцентно меченный зонд для идентификации ДНК аденовируса серотипов 3, 4, 7, 14, 21 методом гибридизационно-флуоресцентной полимеразной цепной реакции в режиме реального времени. Представленные праймеры и зонд имеют следующую структуру:внешний праймер 5'→3'5'-AATGTARTTGGGTCTGTTRGGCAT-3'внутренние праймеры 5'→3'5'-CCCWTCGATGMTGCCCC-3'5'-TCMACGGGYACRAAGCGCA-3'зонд 5'→3'ROX-CCTGTCCGGCGATGTGCAT-BHQ2.Изобретение обладает более высокой гомологией к циркулирующим в настоящее время аденовирусам и позволяет идентифицировать ДНК аденовируса серотипов 3, 4, 7, 14, 21. 3 ил., 1 табл.