FUNCTIONAL COLLAGEN HYDROLYSATE MANUFACTURING METHOD

FIELD: food industry. ^ SUBSTANCE: invention is related to food industry, in particular, to meat processing branch. The method involves collection of meat trimming wastes, removal of visible fat and muscular tissue remains, washing in 30-35C flowing water during 5-10 min; milled in a mincer with 2-3 mm diametre holes, the milled mass treatment with enzyme preparations. At the first stage one applies Neutrase 1.5 MG general proteolytic activity enzyme preparation for removal of ballast substances; to provide for that one prepares a water solution of the preparation and adds it to the milled mass with a water duty of 1:2, the dosage being 5 proteolytic activity units per 1 g of raw material. Treatment proceeds during 2-2.5 h at a temperature of 45-55C and pH=6.8-7.2. After treatment the solid fraction is washed with water for removal of hydrolysis products and the enzyme and separated by way of centrifugation at a 83 rps rotation rate during 5 minutes. Then one proceeds with milling on a homogeniser; added to the milled mass is a water solution of "Food Collagenase" collagenolytic activity enzyme preparation with a water duty of 1:2, taken in a quantity of 6 proteolytic activity units per 1 g of raw material. Treatment proceeds during 2.5-3 h at a temperature of 37-40C and pH=6.8-7.2. Solid fraction is washed with water for removal of hydrolysis products and the enzyme and separated by way of centrifugation at a 83 rps rotation rate. The produced collagen hydrolysate is packaged into polyethylene bags and frozen. ^ EFFECT: method ensures enhancement of the functional collagen product quality and output, technological process intensification, and collagen native structure preservation. ^ 1 tbl, 2 ex Изобретение относится к пищевой промышленности, в частности к мясоперерабатывающей отрасли. Способ предусматривает приемку отходов жиловки мяса, удаление ножом видимых прирезей жировой и мышечной ткани, промывку в проточной воде температурой 30-35°С в течение 5-10 мин; измельчение на волчке с диаметром отверстий 2-3 мм, обработку измельченной массы ферментными препаратами. На первой стадии применяют ферментный препарат общепротеолитического действия Нейтраза 1.5 MG с целью удаления балластных веществ, для чего готовят его водный раствор и вносят в измельченную массу с гидромодулем 1:2 при дозировке 5 ед. протеолитической активности на 1 г сырья, обработку ведут в течение 2-2,5 ч, при температуре 45-55°С и рН 6,8-7,2. После обработки твердую фракцию промывают водой