RECOMBINANT PLASMID pD4spGBD, Escherichia coli STRAIN - RECOMBINANT PROTEIN D4-GBD PRODUCER, RECOMBINANT PROTEIN D4-GBD AND METHOD FOR MAKING THEREOF, METHOD FOR STUDYING PROTEIN D4-GBD FIXATION TO SERUM ANTIBODIES, METHOD FOR MAKING SPECIFIC PROTEIN D4-GBD ANTIBODIES

FIELD: medicine. ^ SUBSTANCE: invention represents plasmid DNA pD4spGBD enabling the expression of a recombinant antigen - protein LigA L. interrogans domain 4 in chimeric protein D4-GBD and 1,3--glucan-binding domain (GBD), and a method for making a based subunit engineered leptospirosis vaccine. ^...

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Hauptverfasser:	SHARAPOVA NATAL'JA EVGEN'EVNA, ANAN'INA JULIJA VASIL'EVNA, GINTSBURG ALEKSANDR LEONIDOVICH, VERKHOVSKAJA LJUDMILA VIKTOROVNA, LUNIN VLADIMIR GLEBOVICH
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	FIELD: medicine. ^ SUBSTANCE: invention represents plasmid DNA pD4spGBD enabling the expression of a recombinant antigen - protein LigA L. interrogans domain 4 in chimeric protein D4-GBD and 1,3--glucan-binding domain (GBD), and a method for making a based subunit engineered leptospirosis vaccine. ^ EFFECT: invention allows ensuring the development of an intensive immune response and high level of antibodies synthesis with no additional adjuvants. ^ 6 cl, 1 dwg, 6 ex Изобретение относится к биотехнологии и представляет собой плазмидную ДНК pD4spGBD, обеспечивающую экспрессию рекомбинантного антигена - домена 4 белка LigA L. interrogans в составе химерного белка D4-GBD с 1,3-β-глюкансвязывающим доменом (GBD), и получения на их основе субъединичной генно-инженерной вакцины против лептоспироза. Изобретение позволяет обеспечивать развитие интенсивного иммунного ответа и высокий уровень синтеза антител без применения дополнительных адъювантов. 6 н.п. ф-лы, 1 ил.