WAY OF REVEALING ANTIBODIES TO ANTIGENS OF HEPATITIS VIRUSES

FIELD: medicine. ^ SUBSTANCE: invention concerns medicine area, namely to diagnostics of infectious diseases and concerns a method of revealing antibodies to antigens of hepatitis viruses. The essence of the way consists in use of an immunochromatographic strip which consists from fiber glass non-sorbing matrix (6) on which the strips containing conjugates of painted particles with monoclonal antibodies to human immunoglobulins G and M (7,8) plastic substrates are placed and immobilised by means of drying, covered with an adhesin layer with low penetrating ability (1) on which a microporous nitrate cellulose membrane with evaporated on a side close to the substrate with a protective polymeric basis (2) is mounted, and admixtures of antigens of viruses of a hepatitis A, B, C (3, 4, 5) are placed perpendicularly to the long side of the strip on the membrane from the non-protected side. The strip is moistened with investigated blood serum and at detection of the painted strip around the strip of antigens of hepatitis A, antibodies to an antigen of a virus of hepatitis A are detected. At detection of the painted strip around the strip of antigens of hepatitis B, antibodies to an antigen of a virus of hepatitis B are detected. At detection of the painted strip around the strip of antigens of hepatitis C, antibodies to an antigen of a virus of hepatitis C are detected, which are formed at linkage of antibodies of blood serum with conjugates of antibodies to immunoglobulins G and M with the painted particles. ^ EFFECT: method allows to tap simultaneously antibodies to antigens of viruses of a hepatitis A, B, C and to define to what type they are specific to. ^ 2 ex, 1 tbl, 1 dwg Изобретение относится к области медицины, а именно к диагностике инфекционных заболеваний и касается способа выявления антител к антигенам вирусов гепатитов. Сущность способа заключается в использовании иммунохроматографического стрипа, который состоит из стекловолоконной несорбирующей матрицы (6), на которую нанесены и иммобилизованы с помощью высушивания полосы, содержащие конъюгаты окрашенных частиц с моноклональными антителами к иммуноглобулинам G и М человека (7, 8) пластиковой подложки, покрытой адгезиновым слоем с низкой проницающей способностью (1), на которой смонтирована микропористая нитратцеллюлозная мембрана с напыленной на прилежащую к подложке сторону защитной полимерной основой (2), причем на мембрану с незащищенной стороны нанесены перпендикулярно длинной стороне стрипа с