METHOD OF PURIFYING HUMAN INTERFERON BETA

FIELD: chemistry, biochemistry. ^ SUBSTANCE: invention relates to biotechnology. Method of purifying human interferon beta from a recombinant culture which contains human interferon beta includes carrying out affinity chromatography and cation-exchange chromatography. Affinity chromatography includes adsorption of the culture containing interferon beta, in a balanced chromatograph column for affinity chromatography, rinsing the column using a liquid buffering solution A with a pH 6.5-7.5, containing 30-60 wt % of propylene-glycol, and liquid buffering solution B with a pH 6.5-7.5, containing 10-30 wt % of propylene-glycol and 1-2 M NaCl, with elusion fraction, containing human interferon beta, with a buffering solution with pH 6.5-7.5, containing 40-60 wt % propylene-glycol and 1-2 M NaCl. Solution obtained is subjected to diafiltration and cation-exchange chromatography is carried out, elute the interferon-containing fraction at a pH 5-7 with the gradient of concentration of NaCl within the limits 0-400 mM followed by concentration and gel-filtration of the product. ^ EFFECT: obtaining interferon beta, characterised with a high level of purity (99%) with the simplification of the technology of separation. ^ 4 cl, 4 dwg, 4 ex