METHOD OF DNA-CHIP OBTAINING
FIELD: medicine; biotechnologies. ^ SUBSTANCE: on a kapron membrane immobilise oligonucleotide probes in which the oligonucleotide sequence is linked to the Poly-t sequence by means of diethylene glycol linker. An immobilisation is performed with the help of ultraviolet irradiation in a dose of 0.08...
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Zusammenfassung: | FIELD: medicine; biotechnologies. ^ SUBSTANCE: on a kapron membrane immobilise oligonucleotide probes in which the oligonucleotide sequence is linked to the Poly-t sequence by means of diethylene glycol linker. An immobilisation is performed with the help of ultraviolet irradiation in a dose of 0.08-1.33 J/cm2. ^ EFFECT: obtaining of a DNA-chip with high superficial capacity and carrying out of the analysis of sequences of DNA with the increased efficiency. ^ 4 dwg, 6 ex |
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