METHOD OF CULTURING STRAIN Rhodococcus rhodochrous M33, WHICH PRODUCES NITRILEHYDRASE

FIELD: chemistry; biotechnology. ^ SUBSTANCE: method of culturing the strain Rhodococcus rhodochrous M33 provides the use of a culture medium, which is based on 12-60 mM phosphate buffer in demineralised water, which meets the cell requirements in phosphor. The value of the pH is kept in the interva...

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Hauptverfasser:	SINGIRTSEV IGOR' NIKOLAEVICH, DEBABOV VLADIMIR GEORGIEVICH, KOZULIN SERGEJ VLADIMIROVICH, LORENTS JOZEF, JANENKO ALEKSANDR STEPANOVICH, VORONIN SERGEJ PETROVICH
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	FIELD: chemistry; biotechnology. ^ SUBSTANCE: method of culturing the strain Rhodococcus rhodochrous M33 provides the use of a culture medium, which is based on 12-60 mM phosphate buffer in demineralised water, which meets the cell requirements in phosphor. The value of the pH is kept in the interval from 5.5 to 9.0. After all the glucose provided has been used then acetic acid and/or its water soluble salt as the new source of carbon. The medium for cultivating the strain includes 7.9 g/l Na2HPO4x12H2O, 1.8 g/l KH2PO4, 0.02-0.04 g/l CoCl2x6H2O, 1.0 g/l MgSO4x7H2O, 0-10 g/l corn steep-water concentrate, 20-90 g/l glucose, 4-20 g/l urea, demineralised water. The medium is used in the following way. Nitrile hydrate activity is 7169 units/millilitre. ^ EFFECT: increased removal of biomass with high nitrilehydrase activity. ^ 19 cl, 3 tbl, 7 ex