METHOD OF QUANTITATIVE DETERMINATION OF PHTHALIC ACID IN BLOOD SERUM

FIELD: medicine; diagnostic techniques. ^ SUBSTANCE: proposed method consists in sampling of 2 cm3 serum, which contains phthalic acid; following acidation with aqueous solution of acetic acid till pH 3; the solid-state extraction at Oasis HLB sorbent is performed with the use of extractant - mixtur...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	KARNAZHITSKAJA TAT'JANA DMITRIEVNA, ZAJTSEVA NINA VLADIMIROVNA, KISLITSINA ANASTASIJA VITAL'EVNA, TEPLOUKHOVA NADEZHDA VLADIMIROVNA, ULANOVA TAT'JANA SERGEEVNA
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	FIELD: medicine; diagnostic techniques. ^ SUBSTANCE: proposed method consists in sampling of 2 cm3 serum, which contains phthalic acid; following acidation with aqueous solution of acetic acid till pH 3; the solid-state extraction at Oasis HLB sorbent is performed with the use of extractant - mixture of methyl and isopropyl alcohol in volume ratio 9:1. Then, the resulted extract is analysed by liquid chromatography, where the mixture of acetonitrile and water in volume ratio 15:85 is used following acidation with aqueous solution of orthophosphoric acid till pH 3. The amount of phthalic acid is determined by using the calibrating plot. ^ EFFECT: precision of phthalic acid test in presence of benzoic acid is increased; test time is reduced. ^ 3 cl, 3 tbl, 2 ex