METHOD FOR DIAGNOSING HAND AND FOOT ONYCHOMYCOSIS LESION

FIELD: medicine. ^ SUBSTANCE: method involves identifying Trichophyton rubrum by applying polymerase chain reaction of DNA separated from skin or nail plate sample incubated in 2-4 M alkaline solution for at least 1 h at 50-65C. The solution is neutralized with 30% HCl acid, the sample is resediment...

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Hauptverfasser:	SOKOLOV GRIGORIJ NIKITICH, TOTOLJAN AREG ARTEMOVICH, ARAVIJSKAJA ELENA ROAL'DOVNA, SOKOLOVSKIJ EVGENIJ VLADISLAVOVICH, KUZNETSOV ALEKSEJ VLADIMIROVICH, CHUKHLOVIN ALEKSEJ BORISOVICH
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	FIELD: medicine. ^ SUBSTANCE: method involves identifying Trichophyton rubrum by applying polymerase chain reaction of DNA separated from skin or nail plate sample incubated in 2-4 M alkaline solution for at least 1 h at 50-65C. The solution is neutralized with 30% HCl acid, the sample is resedimented in 50% ethanol solution at -10 - -20C and dried at 50-65C. The DNA polymerase chain reaction is carried out with synthetic DNA-probes having sequence of TR-1-F (sense): 5'atcgaatctttgaacgcaca'; TR-1-F (antisense): 5'tataaagattggggccaggg 3' highly specific for gene 28 S Trichophyton rubrum RNA. The result being positive, onychomycosis is diagnosed caused by Trichophyton rubrum fungus. ^ EFFECT: high specificity of diagnosis method. ^ 2 dwg, 1 tbl