METHOD FOR INITIAL ISOLATION OF INFLUENZA VIRUS A STRAIN, STRAIN VIRUS A/DUCK/NOVOSIBIRSK/56/05 H5N1 FOR PREPARATION FOR DIAGNOSIS, PROPHYLAXIS AND THERAPEUTICAL AGENTS, AND FOR EVALUATION OF ANTIVIRAL ACTIVITY OF VARIOUS COMPOUNDS

FIELD: medical virology. ^ SUBSTANCE: initial isolation of influenza virus A/H5N1 strain is carried out by contamination of cell culture of porcine embryo kidney. Contaminated culture is incubated in supporting medium 199 with penicillin and streptomycin addition in presence of medium TPCK treated t...

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Hauptverfasser:	VLASOV NIKOLAJ ANATOL'EVICH, ALIPER TARAS IVANOVICH, PRILIPOV ALEKSEJ GENNAD'EVICH, ZABEREZHNYJ ALEKSEJ DMITRIEVICH, KIREEV DMITRIJ EVGEN'EVICH, L'VOV DMITRIJ KONSTANTINOVICH, SHCHELKANOV MIKHAIL JUR'EVICH, DERJABIN PETR GRIGOR'EVICH, NEPOKLONOV EVGENIJ ANATOL'EVICH, GREBENNIKOVA TAT'JANA VLADIMIROVNA
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	FIELD: medical virology. ^ SUBSTANCE: initial isolation of influenza virus A/H5N1 strain is carried out by contamination of cell culture of porcine embryo kidney. Contaminated culture is incubated in supporting medium 199 with penicillin and streptomycin addition in presence of medium TPCK treated tripsin. Disclosed is strain A/duck/Novosibirsk/56/05 H5N1 isolated by abovementioned method. Strain is capable of inducing virus neutralizing protective antibodies and is useful in preparation of test-systems and other diagnosis preparations. ^ EFFECT: strain of high antigen activity. ^ 4 cl, 1 tbl, 4 ex