BINDING OF PATHOLOGICAL FORMS OF PRION PROTEINS

FIELD: medicine, diagnostics. ^ SUBSTANCE: the method for selective binding of aggregating abnormal form of [protein at the presence of nonaggregating normal form of this protein deals with contacting under conditions of selective binding of the material containing the above-mentioned abnormal form and normal form, with a binding agent. Moreover, the binding agent is polyionic material that has got the avidity of binding towards the mentioned aggregating form of the mentioned protein being present in the sample. The conditions of selective binding deal with the presence of a competitive agent in the solution. The competitive agent has got ionic groups that have the least avidity of binding towards abnormal group of this protein in comparison with a binding agent. The above-mentioned binding should be determined qualitatively or quantitatively due to carrying out the immunoassay upon aggregating form of the protein. As binding agents one should apply polyanionic materials (pentosan polysulfate, dextran sulfate and others) or polycationic materials hexadimetrin, polybren and others). Binding agent could be immobilized upon solid medium. Application of the present innovation enables to increase sensitivity and specificity of diagnostic tests to prion diseases. ^ EFFECT: higher efficiency. ^ 37 cl, 1 dwg, 16 ex