DETERMINATION OF BIOLOGICAL ACTIVITY OF TISSUE PREPARATIONS

FIELD: veterinary. ^ SUBSTANCE: claimed method includes preparation of yeast slurry 1:2 in physiological saline and 2 % glucose solution in distilled water. 1 ml of tissue preparation, 1 ml of yeast slurry, and 28 ml of glucose solution are sampled in test tube. The same component wherein tissue preparation is replaced with 1 ml of sterile physiological saline are sampled in control tube. Tubes are sealed with closure with glass tubes ends of which are dropped down in bulb. 1 drop of liquid is sampled from each tube, placed into chamber, and yeast cell amount is calculated. Further chamber is placed into thermostat at temperature of 33-35°C and held for 60-90 min. After agitation 1 drop of solution is simultaneously sampled from control and test tubes and yeast cell amount is calculated. Activity of tissue preparation is evaluated based on volume of released carbon dioxide and amount of yeast cells in test and control tubes (before and after experiment). ^ EFFECT: accelerated method of decreased cost and increased accuracy. ^ 3 tbl, 5 ex