DEVICE FOR SIMULTANEOUS REAL-TIME SCALE INSPECTION OF MULTIPLICITY OF AMPLIFICATIONS OF NUCLEIC ACID

FIELD: qualitative and quantitative inspection of nucleic acids. ^ SUBSTANCE: device can be used in medical practice when treating infectious, oncology and genetic diseases of human and animals, in research centers for molecular-biological and genetic surveys as well as for genes monitoring. Device...

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Hauptverfasser: VARLAMOV DMITRIJ ALEKSANDROVICH, FESENKO VLADIMIR ANATOL'EVICH, CHERNYSHEV ANDREJ VLADIMIROVICH, MARAKUSHIN NIKOLAJ FEDOROVICH, PETROV ALEKSANDR IVANOVICH, SKOBLILOV EVGENIJ JUR'EVICH, KONOVALOV SERGEJ VLADIMIROVICH, SOKOLOV VALERIJ NIKOLAEVICH, PETRJAKOV ALEKSANDR OLEGOVICH, ALEKSEEV JAKOV IGOREVICH, KUROCHKIN VLADIMIR EFIMOVICH
Format: Patent
Sprache:eng ; rus
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Zusammenfassung:FIELD: qualitative and quantitative inspection of nucleic acids. ^ SUBSTANCE: device can be used in medical practice when treating infectious, oncology and genetic diseases of human and animals, in research centers for molecular-biological and genetic surveys as well as for genes monitoring. Device has thermal cycler with heat-conducting member, thermal cap, temperature mode automatic control device and optical system. There are hollows in heat-conducting member, which hollows intend for test tubes filled with reaction mixtures. Optical system has radiation source, fiber-optic wave-guides for transmitting excitation light from source and for radiation of fluorescence from test tubes, and detector. Light guides are made in form of optical fibers; central part and peripheral part for collecting fluorescence are disposed coaxial. Central part of light guide is aperture mated with amount of reaction mixture in test tubes. ^ EFFECT: maximal usage of light flux for excitation of reaction mixture; reduced of light loss in test-tube filled with reaction mixture; improved sensitivity; higher precision of analysis. ^ 6 cl, 3 dwg