METHOD FOR IMMUNOFERMENT DETECTION OF ANTIGENS

FIELD: biotechnology, analytical chemistry. ^ SUBSTANCE: claimed method includes providing of complexes between antigen molecules and specific antibodies on carrier surface, wherein said complexes are disclosed by addition of enzyme label thereto followed detection thereof based on formation of enzy...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	ZHERDEV ANATOLIJ VITALIEVICH, STREMOVSKIJ OLEG ANATOL'EVICH, DZANTIEV BORIS BORISOVICH, URUSOV ALEKSANDR EVGEN'EVICH, BALANDIN TARAS GENNADIEVICH, EHDEL'VEJS EHVELINA FEDOROVNA, ERMOLENKO DMITRIJ NIKOLAEVICH, DEEV SERGEJ MIKHAJLOVICH
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	FIELD: biotechnology, analytical chemistry. ^ SUBSTANCE: claimed method includes providing of complexes between antigen molecules and specific antibodies on carrier surface, wherein said complexes are disclosed by addition of enzyme label thereto followed detection thereof based on formation of enzyme reaction product. Enzyme label addition is carried out by two protein interaction, namely bacterial ribonucleaze barnase and barstare, which is inhibitor thereof, wherein either of the two is added to immunoreagent, and the other one is added to enzyme label. Abovementioned complexes have high affinity, specificity, and binding constant of 1014 M-1. ^ EFFECT: new method for antigen detection. ^ 6 dwg, 2 ex