METHOD FOR RECOMBINANT PROTEIN PURIFICATION

FIELD: biotechnology, in particular recombinant protein purification from microorganism strains-producers, vaccine production. ^ SUBSTANCE: claimed method includes application of nanoparticles of bivalent metals having particle diameter of 20-100 nm. Recombinant protein binding is carried out direct...

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Bibliographische Detailangaben
Hauptverfasser:	KUSKOV MIKHAIL LEONIDOVICH, LAZAREV VASILIJ NIKOLAEVICH, GOVORUN VADIM MARKOVICH, ZHIGACH ALEKSEJ NIKOLAEVICH, LEJPUNSKIJ IL'JA OVSEEVICH, NIKOLAEV EVGENIJ NIKOLAEVICH
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	CHEMISTRY METALLURGY ORGANIC CHEMISTRY PEPTIDES
Online-Zugang:	Volltext bestellen
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Beschreibung
Zusammenfassung:	FIELD: biotechnology, in particular recombinant protein purification from microorganism strains-producers, vaccine production. ^ SUBSTANCE: claimed method includes application of nanoparticles of bivalent metals having particle diameter of 20-100 nm. Recombinant protein binding is carried out directly in coarse cell lysates, and particle separation from non-bonded proteins is carried out by using magnetic separation. ^ EFFECT: method of decreased cost. ^ 2 dwg, 1 ex