METHOD FOR PRODUCING ERYTHROCYTIC ANTIGEN FOR PREDICTING VIBRIOSIS IN FISH

FIELD: biotechnology, pisciculture. ^ SUBSTANCE: the innovation deals with preparing nutritive media, inoculation material and obtaining sensitin out of the strains Vibrio anguillarum N2 and VUR-19 followed by sensitization of ram's erythrocytes. Moreover, it is necessary to apply 20%-suspensio...

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Hauptverfasser:	LITVINOV OLEG BORISOVICH, SHUMILOV KONSTANTIN VASIL'EVICH, APAL'KIN VIKTOR ALEKSANDROVICH, BEZGACHINA TAT'JANA VLADIMIROVNA, MEL'NIK NIKOLAJ VASIL'EVICH, SKLJAROV OLEG DMITRIEVICH, ELESEEV ANATOLIJ KONSTANTINOVICH, BONDARENKO VIKTOR ZINOV'EVICH
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES TESTING
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Beschreibung
Zusammenfassung:	FIELD: biotechnology, pisciculture. ^ SUBSTANCE: the innovation deals with preparing nutritive media, inoculation material and obtaining sensitin out of the strains Vibrio anguillarum N2 and VUR-19 followed by sensitization of ram's erythrocytes. Moreover, it is necessary to apply 20%-suspension of formalinized erythrocytes upon phosphate-buffer solution at pH being 7.0-7.4. Sensitization should be carried out upon water bath in two stages. Erythrocytes and sensitin should be taken at the ratio of 1:0.3-1:0.35. ^ EFFECT: higher efficiency. ^ 2 ex