METHOD FOR PREPARING VACCINE AGAINST ENTEROCOCCUS INFECTION IN NUTRIAS

FIELD: veterinary medicine. ^ SUBSTANCE: the suggested vaccine as an antigen contains the suspension of cells of pure culture of Streptococcus fecalis agent obtained due to selecting affected organs in dead nutrias from local epizootic focus to prepare the suspension followed by inoculation onto dif...

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Hauptverfasser: VORONOVA IRINA SERGEEVNA, SHEVCHENKO ANNA ALEKSANDROVNA, SHEVCHENKO ALEKSANDR ALEKSEEVICH, SHEVCHENKO LJUDMILA VASIL'EVNA
Format: Patent
Sprache:eng ; rus
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Zusammenfassung:FIELD: veterinary medicine. ^ SUBSTANCE: the suggested vaccine as an antigen contains the suspension of cells of pure culture of Streptococcus fecalis agent obtained due to selecting affected organs in dead nutrias from local epizootic focus to prepare the suspension followed by inoculation onto differentially-diagnostic media; then one should isolate pure culture of Streptococcus fecalis agent to be grown in beef-infusion-peptone broth at addition of 0.2%-glucose to achieve the concentration of about 5-6 bln. microbial cells/cu. cm followed by inactivation due to introducing formalin up to 0.4-0.6%-final concentration to be kept at 37 C for about 72-96 h and aluminum hydroxide at the quantity of 20% against the volume of the culture to be thoroughly mixed, packed and sealed. The vaccine is safe and highly efficient. ^ EFFECT: higher efficiency. ^ 1 ex, 1 tbl