METHOD FOR PRODUCTION OF BACTERIAL PREPARATION FOR MEAT PRODUCT MANUFACTURING
FIELD: biotechnology, in particular meat product manufacturing. ^ SUBSTANCE: claimed method includes deep cultivation of Paracoccus denitrificants K-3 denitrification strain under aeration condition in nutrient medium containing organic amine nitrogen source, yeast-based nitrogen source, glucose, ma...
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Zusammenfassung: | FIELD: biotechnology, in particular meat product manufacturing. ^ SUBSTANCE: claimed method includes deep cultivation of Paracoccus denitrificants K-3 denitrification strain under aeration condition in nutrient medium containing organic amine nitrogen source, yeast-based nitrogen source, glucose, magnesium sulfate, manganese sulfate, and water; cell mass separation, concentration, blending with protective medium, freeze drying, and blending of bacterial concentrate with filler. Nutrient medium additionally contains sodium chloride, monosubstituted sodium phosphate, disubstituted potassium phosphate, and potassium nitrite; alkaline hydrolyzate of cattle blood, and redox potential reducing component. Pancreatic casein hydrolyzate containing 600 mg% of amine nitrogen or pancreatic defatted milk hydrolyzate containing 600 mg% of amine nitrogen are used as amine nitrogen source, and baking yeast autolyzate is used as yeast-based nitrogen source in specific component ratio. Preparation of present invention holds denitrification activity during long time (8-9 months) and assures low sodium nitrite concentration in finished meat products. ^ EFFECT: convenient bacterial preparation; meat products of improved characteristics. ^ 2 cl, 1 dwg |
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