METHOD FOR MICROBIOLOGICAL SYNTHESIS OF LACTIC ACID AND RECOMBINANT STRAIN OF YEAST SCHIZOSACCHAROMYCES POMBE FOR ITS REALIZATION

METHOD FOR MICROBIOLOGICAL SYNTHESIS OF LACTIC ACID AND RECOMBINANT STRAIN OF YEAST SCHIZOSACCHAROMYCES POMBE FOR ITS REALIZATION

FIELD: biotechnology, microbiology, genetic engineering. ^ SUBSTANCE: invention relates to methods for preparing lactic acid. Lactic acid is prepared by culturing the recombinant strain of yeast of genus Schizosaccharomyces as a producer of lactic acid comprising at least one foreign lactate dehydro...

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Hauptverfasser: JUZBASHEV TIGRAN VLADIMIROVICH, DEBABOV VLADIMIR GEORGIEVICH, RYBAKOV JURIJ ALEKSANDROVICH, TOKAREVA NADEZHDA GEORGIEVNA, RAJNINA EVGENIJA ISAAKOVNA, VELIKAJA MARINA ALEKSANDROVNA, AGRANOVICH ANNETA MIKHAJLOVNA, SINEOKIJ SERGEJ PAVLOVICH, VUSTIN MIKHAIL MIKHAJLOVICH
Format: Patent
Sprache:eng ; rus
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:FIELD: biotechnology, microbiology, genetic engineering. ^ SUBSTANCE: invention relates to methods for preparing lactic acid. Lactic acid is prepared by culturing the recombinant strain of yeast of genus Schizosaccharomyces as a producer of lactic acid comprising at least one foreign lactate dehydrogenase gene. The recombinant strain of yeast Schizosaccharomyces pombe VKPM Y-3127 is prepared by using the strain Schizosaccharomyces pombe VKPM Y-285 as a recipient and transformation of the strain is carried out with plasmid DNA carrying IdhA gene from fungus Rhizopus oryzae. Invention provides carrying out synthesis of lactic acid at low pH values and show capacity for producing lactic acid at pH 4.0 up to the concentration of lactic acid in medium 80-100 g/l. ^ EFFECT: improved preparing method. ^ 3 cl, 5 dwg, 2 tbl, 4 ex