METHOD FOR PRODUCTION OF BIOACTIVE SUPPLEMENT

FIELD: biotechnology, in particular production of bioactive supplements useful in treatment and prophylaxis of various gastrointestinal diseases in any kind of agriculture animals and poultry. ^ SUBSTANCE: claimed method includes introducing of lactobacterium cultures Lactobacillus acidophilus and L...

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Hauptverfasser:	DUBENKO ALEKSANDR JAKOVLEVICH, SURMILO ALEKSEJ PETROVICH, SAJAN VLADIMIR SHAVASHOVICH, ZAERKO VIKTOR IVANOVICH
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	FIELD: biotechnology, in particular production of bioactive supplements useful in treatment and prophylaxis of various gastrointestinal diseases in any kind of agriculture animals and poultry. ^ SUBSTANCE: claimed method includes introducing of lactobacterium cultures Lactobacillus acidophilus and Lactobacillus delbrueskii subsp. Bulgaricus in equal ratio in broth containing lightened milky whey, yeast extract, manganese sulfate, and potassium phosphate. Lactobacterium biomass is grown by deep cultivation process at 44±1°C. During cultivation medium pH is adjusted and double neutralization is carried out. After 2-3 h form cultivation starting Lactobacillus lactic subsp. lactic III Defatted milk, 40 % glucose solution are introduced into broth followed by further cultivation. Before cultivation finishing sodium citrate is introduced and mixture is cooled. Cooled biomass is centrifuged ad separated. Concentrated biomass is blended with sucrose/gelatin protective medium and potassium iodide, pre-packed in penicillin flacks, and freeze dehydrated. ^ EFFECT: inexpensive harmless and convenient preparation having high antagonistic properties against pathogenic microflora and increase concentration of healthy cells. ^ 3 ex