TEST CARRIER AND METHOD FOR RAPID MEASUREMENT OF MICROORGANISMS ABSOLUTE CONCENTRATION IN BIOSUBSTRATE BY THEIR PHOTOLUMINESCENCE (VARIANTS)
FIELD: microbiology, optics. ^ SUBSTANCE: invention relates to investigations of materials by assay of their physical or chemical properties using optical devices and to systems wherein material is excited by optical agents resulting to it luminescence. Invention proposes a test carrier as a centrif...
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Sprache: | eng ; rus |
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Zusammenfassung: | FIELD: microbiology, optics. ^ SUBSTANCE: invention relates to investigations of materials by assay of their physical or chemical properties using optical devices and to systems wherein material is excited by optical agents resulting to it luminescence. Invention proposes a test carrier as a centrifugal tube. Carrier is separated for upper and bottom cavities by partition. Volume of lower cavity is 0.1 of tube volume. A hole is made in partition near a wall. The constructive decision of partition provides efflux of sample from lower cavity with minimal overcoming the combined forces of wetting and surface tension. Also, invention proposes methods/variants for rapid measurement of absolute concentration of microorganisms in biosubstrate by their photoluminescence. Methods involve using fluorescent or phosphorescent measuring device and above said test carrier. Methods provides increasing rate and precision of assay, to use serial measuring devices and to carry out measurement of the concentration of particles in substrate with another specific gravity value as compared with that of liquid in substrate. Invention can be used in food and biotechnological industry for determination of absolute concentration of microorganisms in different substrates. ^ EFFECT: improved method for assay, valuable properties of carrier. ^ 2 tbl, 1 dwg |
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