RECOMBINANT PLASMID DNA PCDNA-TCI PROVIDING EXPRESSION OF ARTIFICIAL GENE TCI IN EUCARYOTIC CELLS AND RECOMBINANT ATTENUATED STRAIN OF BACTERIUM SALMONELLA ENTERITIDIS E-23 PC-DNA-TCI AS CANDIDATE FOR CONSTRUCTION OF LIVE DNA-VACCINE AGAINST HUMAN IMMUNODEFICIENCY VIRUS

FIELD: biotechnology, genetic engineering, molecular biology. ^ SUBSTANCE: invention proposes recombinant DNA pcDNA-TCI that has molecular mass 4.3 x 103 kDa and size 6 657 nucleotide pairs. The proposed recombinant plasmid DNA provides expression of artificial gene TCI comprising cytotoxic T-cellul...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	IGNAT'EV G.M, SANDAKHCHIEV L.S, SEREGIN S.V, NEKRASOVA N.A, IL'ICHEV A.A, BAZHAN S.I, BOJCHENKO M.N, BABKINA I.N, VOROB'EV A.A, KARPENKO L.I, AGAFONOV A.P, BELAVIN P.A, DANILJUK N.K
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	FIELD: biotechnology, genetic engineering, molecular biology. ^ SUBSTANCE: invention proposes recombinant DNA pcDNA-TCI that has molecular mass 4.3 x 103 kDa and size 6 657 nucleotide pairs. The proposed recombinant plasmid DNA provides expression of artificial gene TCI comprising cytotoxic T-cellular epitopes of HIV-1 in eucaryotic cells. Also, invention proposes recombinant attenuated strain of bacterium Salmonella enteritidis E-23 pcDNA-TCI. The proposed strain is obtained by genetic transformation of the strain Salmonella enteritidis E-23 with plasmid pcDNA-TCI. The strain provides delivery DNA-vaccine in eucaryotic cells as the recombinant plasmid DNA pcDNA-TCI. Proposed groups of inventions provide inducing the expressed specific humoral and cellular immune response to HIV-1 in body. Proposed group of inventions can be used in medicine and virology for construction of live DNA-vaccine against HIV-1. ^ EFFECT: valuable biological and medicinal properties of strain and vaccine. ^ 2 cl, 3 dwg, 3 tbl, 5 ex