METHOD FOR ASSAY OF ANTIRADICAL ACTIVITY OF SUBSTANCES IN VITRO

FIELD: biotechnology. ^ SUBSTANCE: invention can be used for assay of antiradical activity of substances by their ability to interact with radicals OH. Determination of value of constant rate reaction of substance with radicals OH (kOH) is carried out under conditions of gamma-irradiation using p-ni...

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Bibliographische Detailangaben
Hauptverfasser:	SAKHAROVA V.A, REBROV L.B, BYKOV V.A, KONDAKOVA N.V, RIPA N.V, KOLKHIR V.K
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	FIELD: biotechnology. ^ SUBSTANCE: invention can be used for assay of antiradical activity of substances by their ability to interact with radicals OH. Determination of value of constant rate reaction of substance with radicals OH (kOH) is carried out under conditions of gamma-irradiation using p-nitrosodimethylaniline an aqueous solution as a comparison standard. Substances with value kOH above (4-5) x 109 l/mole x s are selected. Then ratios of equal-damaging doses are determined successively at gamma-irradiation of trypsin, DNA and erythrocytes in the presence of selected substance and without it and antiradical activity of analyzed substance is evaluated by comparative analysis. Invention provides carrying out the quantitative evaluation of antiradical activity of biologically active substances (BAS), to compare antiradical and antioxidant effects of BAS and to make reliable conclusions about properties of BAS. ^ EFFECT: improved assay method. ^ 1 tbl