TRANSFORMED STRAIN OF YEAST HANSENULA POLYMORPHA AS PRODUCER OF RECOMBINANT SURFACE ANTIGEN OF HEPATITIS VIRUS B-HBSAG/ADW

FIELD: genetic engineering, molecular biology, microbiology. ^ SUBSTANCE: invention can be used for the development of microbiological yeast producer providing high yield of recombinant antigen of hepatitis virus B of serotype adw. By carrying out genetic-engineering procedures the yeast strain Hans...

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Hauptverfasser:	MEL'NIKOV V.A, JAKOVLEVA I.M, BORISOVA V.N, BUDANOV M.V
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	FIELD: genetic engineering, molecular biology, microbiology. ^ SUBSTANCE: invention can be used for the development of microbiological yeast producer providing high yield of recombinant antigen of hepatitis virus B of serotype adw. By carrying out genetic-engineering procedures the yeast strain Hansenula polymorpha VKPM Y-2412 is prepared that is transformed with DNA sequence encoding HBsAg/adw fused with gene MOX promoter and with gene TRP3 and representing the producer of recombinant surface antigen of hepatitis B virus. Invention allows preparing enhanced levels in synthesis of antigen and simplifying methods for preparing cultures of high density. ^ EFFECT: valuable properties of yeast strain. ^ 8 ex