METHOD FOR ISOLATING RIBONUCLEIC ACIDS
FIELD: biotechnology, molecular biology, preparative biochemistry. ^ SUBSTANCE: invention relates to methods for isolation of ribonucleic acids (RNAs) and can be used in molecular-biological investigations. Method involves incubation of biological sample with small-dispersed meshed glass treated wit...
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| creator | VLASOV V.V LAKTIONOV P.P SIMONOV P.A RYKOVA E.JU TAMKOVICH S.N |
| description | FIELD: biotechnology, molecular biology, preparative biochemistry. ^ SUBSTANCE: invention relates to methods for isolation of ribonucleic acids (RNAs) and can be used in molecular-biological investigations. Method involves incubation of biological sample with small-dispersed meshed glass treated with 3.5-7% hydrofluoric acid solution preliminary for 2-6 h in buffer solution containing chaotropic agent, separation of sorbent and sorbent-bound RNA by centrifugation, washing out sorbent and elution of RNAs from sorbent with buffer solution containing 5-50 mM of hydrocarbonate ions (HCO3) and 5-10 mM of EDTA at pH 8.0-10.0. Method provides reducing time process, enhancing RNAs yield and to provide isolation of both low-molecular and high-molecular RNA fractions. ^ EFFECT: improved isolating method. ^ 3 cl, 5 dwg, 2 tbl, 3 ex |
| format | Patent |
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Method involves incubation of biological sample with small-dispersed meshed glass treated with 3.5-7% hydrofluoric acid solution preliminary for 2-6 h in buffer solution containing chaotropic agent, separation of sorbent and sorbent-bound RNA by centrifugation, washing out sorbent and elution of RNAs from sorbent with buffer solution containing 5-50 mM of hydrocarbonate ions (HCO3) and 5-10 mM of EDTA at pH 8.0-10.0. 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Method involves incubation of biological sample with small-dispersed meshed glass treated with 3.5-7% hydrofluoric acid solution preliminary for 2-6 h in buffer solution containing chaotropic agent, separation of sorbent and sorbent-bound RNA by centrifugation, washing out sorbent and elution of RNAs from sorbent with buffer solution containing 5-50 mM of hydrocarbonate ions (HCO3) and 5-10 mM of EDTA at pH 8.0-10.0. 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Method involves incubation of biological sample with small-dispersed meshed glass treated with 3.5-7% hydrofluoric acid solution preliminary for 2-6 h in buffer solution containing chaotropic agent, separation of sorbent and sorbent-bound RNA by centrifugation, washing out sorbent and elution of RNAs from sorbent with buffer solution containing 5-50 mM of hydrocarbonate ions (HCO3) and 5-10 mM of EDTA at pH 8.0-10.0. Method provides reducing time process, enhancing RNAs yield and to provide isolation of both low-molecular and high-molecular RNA fractions. ^ EFFECT: improved isolating method. ^ 3 cl, 5 dwg, 2 tbl, 3 ex</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE |
| title | METHOD FOR ISOLATING RIBONUCLEIC ACIDS |
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