METHOD FOR ISOLATING RIBONUCLEIC ACIDS

FIELD: biotechnology, molecular biology, preparative biochemistry. ^ SUBSTANCE: invention relates to methods for isolation of ribonucleic acids (RNAs) and can be used in molecular-biological investigations. Method involves incubation of biological sample with small-dispersed meshed glass treated wit...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	VLASOV V.V, LAKTIONOV P.P, SIMONOV P.A, RYKOVA E.JU, TAMKOVICH S.N
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	FIELD: biotechnology, molecular biology, preparative biochemistry. ^ SUBSTANCE: invention relates to methods for isolation of ribonucleic acids (RNAs) and can be used in molecular-biological investigations. Method involves incubation of biological sample with small-dispersed meshed glass treated with 3.5-7% hydrofluoric acid solution preliminary for 2-6 h in buffer solution containing chaotropic agent, separation of sorbent and sorbent-bound RNA by centrifugation, washing out sorbent and elution of RNAs from sorbent with buffer solution containing 5-50 mM of hydrocarbonate ions (HCO3) and 5-10 mM of EDTA at pH 8.0-10.0. Method provides reducing time process, enhancing RNAs yield and to provide isolation of both low-molecular and high-molecular RNA fractions. ^ EFFECT: improved isolating method. ^ 3 cl, 5 dwg, 2 tbl, 3 ex