METHOD FOR ISOLATION OF VITAMIN B12

FIELD: microbiological industry, vitamins. SUBSTANCE: invention relates to method for isolation of vitamin B12 from bacterial mass. Method involves concentrating ammonia eluate using nanofiltration that is carried out in regimen of tangential (flow-type) filtration in value of transmembrane pressure...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	USTJUZHANINA I.JU, KAMINSKAJA M.I, PSHENICHNIKOV V.G, VAKHRAMEEVA G.A
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL SEPARATION SPIRITS TRANSPORTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	FIELD: microbiological industry, vitamins. SUBSTANCE: invention relates to method for isolation of vitamin B12 from bacterial mass. Method involves concentrating ammonia eluate using nanofiltration that is carried out in regimen of tangential (flow-type) filtration in value of transmembrane pressure from 0.02 to 0.05 MPa that provides the filtration rate of ammonia eluate in carrying out the concentration process from 15 to 20 l/m2.h. Nominal threshold for retention of nanofiltrating membrane is 400 Da that provides practically complete retention of vitamin B12. Method provides the enhancement of yield and quality of preparation, to simplify technological process, to reduce raw and energy consumptions due to exclusions of the following stages: adsorption of vitamin of activated carbon, desorption from carbon with isopropyl alcohol an aqueous solution, distillation off of alcohol and evaporation of an aqueous concentrate. EFFECT: improved isolating method. 2 ex