METHOD OF DETERMINATION OF BLOOD LIPOPROTEID FRACTIONS IN PATIENTS WITH HEART ISCHEMIC DISEASE

FIELD: medicine, cardiology, biochemistry. SUBSTANCE: method of separation of blood lipoproteid fractions in patients with heart ischemic disease involves electrophoresis in agarose gel, treatment of patient serum sample blood with dye sudan B solution in darkness for 1 h, addition of agarose soluti...

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Hauptverfasser:	KARPOV R.S, KANSKAJA N.V, KANSKIJ A.V, PAKHRJAEV E.N, PEROVA N.V
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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Beschreibung
Zusammenfassung:	FIELD: medicine, cardiology, biochemistry. SUBSTANCE: method of separation of blood lipoproteid fractions in patients with heart ischemic disease involves electrophoresis in agarose gel, treatment of patient serum sample blood with dye sudan B solution in darkness for 1 h, addition of agarose solution, addition of agarose heated up to 55 C into gel well, the following electrophoregram fixing, their drying and densitometry. In treatment of serum sample with dye sudan B solution sample is placed in dark thermostat and heated mixture is added into well with volume 4 x 20 x 10 mm. Method allows to detect fraction LA(a) and can be used for diagnosis in cardiology. EFFECT: enhanced sensitivity and precision of method. 8 dwg