SURFACE ANCHOR VECTOR (VERSIONS), STRAIN ESCHERICHIA COLI TRANSFORMED BY VECTOR (VERSIONS), METHOD OF EXPRESSION OF FOREIGN PROTEINS ON CELLULAR SURFACE

SURFACE ANCHOR VECTOR (VERSIONS), STRAIN ESCHERICHIA COLI TRANSFORMED BY VECTOR (VERSIONS), METHOD OF EXPRESSION OF FOREIGN PROTEINS ON CELLULAR SURFACE

biotechnology, molecular biology, protein engineering. SUBSTANCE: surface anchor vector comprises segments of gene encoding protein that induces formation of ice microcrystals (INP) from Pseudomonas syringe and novel restriction sites for insertion foreign genes by C-end of INP. Gene segments compri...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: DZHUNG KHEUNG CHAE, PARK SEUNG KHVAN, PARK EUNG KHOON, PAN DZHAE GU, KHAN MOON KHI
Format: Patent
Sprache:eng ; rus
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMES
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:biotechnology, molecular biology, protein engineering. SUBSTANCE: surface anchor vector comprises segments of gene encoding protein that induces formation of ice microcrystals (INP) from Pseudomonas syringe and novel restriction sites for insertion foreign genes by C-end of INP. Gene segments comprise N-terminal secretion signal, C-terminal targeting signal and can involve central repeating domain. Gene encoding carboxymethylcellulase or levansucrase is cloned into vector, vector is transformed into Escherichia coli cells and transformed strain is obtained. Expression of protein on cellular surface is performed by the transformed strain culturing. Invention ensures to obtain foreign proteins on surface of cells. EFFECT: improved method of expression and proteins preparing. 14 cl, 12 dwg, 13 ex