RECOMBINANT PLASMID DNA PTRC TE-LEP ENCODING POLYPEPTIDE WITH HUMAN LEPTIN PROPERTY AND STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF POLYPEPTIDE WITH HUMAN LEPTIN PROPERTY

biotechnology, genetic engineering. SUBSTANCE: recombinant plasmid DNA comprises cDNA of processed form of human leptin, Escherichia coli trc-promoter and synthetic site as an enhancer of translation of bacteriophage T7 gene 10 which determining biosynthesis of polypeptide showing properties of huma...

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Hauptverfasser:	ALIEV T.K, PANKOV JU.A, KARPOVA S.K, VARFOLOMEEV S.D, KOROBKO V.G, KRIVTSOV V.F
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	biotechnology, genetic engineering. SUBSTANCE: recombinant plasmid DNA comprises cDNA of processed form of human leptin, Escherichia coli trc-promoter and synthetic site as an enhancer of translation of bacteriophage T7 gene 10 which determining biosynthesis of polypeptide showing properties of human leptin. The strain Escherichia coli W3110/pTrcTE-Lep transformed with the prepared plasmid provides synthesis of this polypeptide at the expression level 43% of total cellular protein, not less, at concentration isopropyl-beta-D-thiogalactopyranoside as an inductor 0.05 mM. EFFECT: increased yield of polypeptide biosynthesis, simultaneous decrease of amount of inductor added. 2 cl, 5 dwg, 6 ex