METHOD OF ISOLATION OF CARBOXYPEPTIDASE B

biochemistry, enzymology. SUBSTANCE: invention relates to method of isolation of carboxypeptidase B from porcine pancreas and can be used in producing genetic engineering materials in biotechnology. Method of isolation of carboxypeptidase B involves extraction, precipitation with ammonium sulfate an...

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Hauptverfasser: GORDEEVA E.A, NOKEL' E.A, ZINCHENKO A.A, TOLSTOV D.N, MIROSHNIKOV A.I, BAIRAMASHVILI D.I
Format: Patent
Sprache:eng ; rus
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Zusammenfassung:biochemistry, enzymology. SUBSTANCE: invention relates to method of isolation of carboxypeptidase B from porcine pancreas and can be used in producing genetic engineering materials in biotechnology. Method of isolation of carboxypeptidase B involves extraction, precipitation with ammonium sulfate and purification and these stages are performed in buffer solutions containing 1-10 mM of L-arginine. Purification stage is carried out by chromatography method on hydroxyapatite at first and then on DEAE-Sepharose as sorbents. EFFECT: improved method of isolation, increased yield of carboxypeptidase B. 8 ex