METHOD OF DETECTION OF CATTLE VIRAL DIARRHEA VIRUS (MUCOSA DISEASE) USING SPECIFIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION

molecular biology, veterinary science. SUBSTANCE: invention relates to method that can be used for detection of cattle viral diarrhea. Method involves synthesis of oligonucleotide primers 5'-TAG-ATT-CCA-GAC-CGA-CCC-3' and 5'-GGA-AGG-GAT-ACA-ACG-GGC-3' and synthesis of complementa...

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Hauptverfasser:	ORESHKOVA S.F, DONCHENKO A.S, SEMENIKHIN V.I, CHEKISHEV V.M, IL'ICHEV A.A, JURIK S.A
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	molecular biology, veterinary science. SUBSTANCE: invention relates to method that can be used for detection of cattle viral diarrhea. Method involves synthesis of oligonucleotide primers 5'-TAG-ATT-CCA-GAC-CGA-CCC-3' and 5'-GGA-AGG-GAT-ACA-ACG-GGC-3' and synthesis of complementary DNA on DNA matrix. Then amplification of synthesized DNA is carried out by method of polymerase chain reaction and polymerase chain reaction products size is analyzed. Proposed method ensures to differentiate cattle viral diarrhea virus from the close-related classical porcine plague virus. EFFECT: improved method of virus detection. 1 tbl, 1 ex