METHOD OF PREPARING GENUS-SPECIFIC LEPTOSPIRA ANTIGEN

microbiology. SUBSTANCE: method involves culturing one or complex of Leptospira pathogenic strains, disintegration of bacterial mass by deep freezing of suspension followed by its extrusion, centrifugation, extraction of sediment with 2.5% sodium chloride solution, centrifugation and precipitation o...

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Bibliographische Detailangaben
Hauptverfasser:	AFANAS'EV E.N, TJUMENTSEVA I.S, BINATOVA V.V, EFREMENKO V.I, POPOV V.A
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	microbiology. SUBSTANCE: method involves culturing one or complex of Leptospira pathogenic strains, disintegration of bacterial mass by deep freezing of suspension followed by its extrusion, centrifugation, extraction of sediment with 2.5% sodium chloride solution, centrifugation and precipitation of the first and the second supernatants with ammonium sulfate solution. Invention can be used in biotechnology for preparing diagnostic preparations. EFFECT: increased specific activity of antigen, increased yield, decreased cost of ready product.