METHOD OF MUCOVISCIDOSIS DIAGNOSIS

medicine, molecular biology. SUBSTANCE: proposed method involves isolation of genome DNA from 5-10 ml of peripheral blood by conventional procedure using proteinase K followed by extraction with mixture phenol/chloroform. For amplification of normal allele direct primer is used: 5'-GGCAC-CATTA-...

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Hauptverfasser:	JUDIN N.S, ROMASHCHENKO A.G, VOEVODA M.I, KOBZEV V.F
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	medicine, molecular biology. SUBSTANCE: proposed method involves isolation of genome DNA from 5-10 ml of peripheral blood by conventional procedure using proteinase K followed by extraction with mixture phenol/chloroform. For amplification of normal allele direct primer is used: 5'-GGCAC-CATTA-AAGAA-AATAT-CATCTT-3' terminating at 3'-end by trinucleotide CTT that is absent in mutant allele. For amplification of allele with deletion direct primer is used: 5'-GGCAC-CATTA-AAGAA-AATAT-CATTG-G-3' without CTT and terminating by the next trinucleotide TGG at 3'-end. As reverse primer for amplification of both alleles primer is used: 5'-CATTC-ACAGT-AGCTT-ACCCA-3'. EFFECT: simplified, precise method, enhanced safety of diagnosis. 2 cl, 1 dwg, 2 ex