STRAIN OF CULTURED MURINE HYBRIDOMA CELLS USED FOR PREPARING MONOCLONAL ANTIBODIES RAISED TO NUCLEOLUS ANTIGEN ASSOCIATED WITH CELLULAR PROLIFERATION

STRAIN OF CULTURED MURINE HYBRIDOMA CELLS USED FOR PREPARING MONOCLONAL ANTIBODIES RAISED TO NUCLEOLUS ANTIGEN ASSOCIATED WITH CELLULAR PROLIFERATION

FIELD: biotechnology, medicine. SUBSTANCE: hybridoma is obtained by fusion of murine splenocytes immunized with nuclear fraction from cells of beta-lymphoblastoid strain Daudi with murine myeloma cells. Hybridoma produces monoclonal antibodies directed to nucleolus antigen (molecular mass is 35 and...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: MALASHENKO O.S, BULYCHEVA T.I, DUDNIK O.A
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:FIELD: biotechnology, medicine. SUBSTANCE: hybridoma is obtained by fusion of murine splenocytes immunized with nuclear fraction from cells of beta-lymphoblastoid strain Daudi with murine myeloma cells. Hybridoma produces monoclonal antibodies directed to nucleolus antigen (molecular mass is 35 and 43 kDa) that is detected in proliferating cells of different species, tissue and strain belonging. Invention ensures to study the process of cellular proliferation in malignant diseases and can be used for identification of proliferating cells. EFFECT: strain indicated above, improved method of hybridoma preparing.