METHOD OF PURIFICATION OF HUMAN RECOMBINANT ERYTHROPOIETIN

FIELD: biotechnology. SUBSTANCE: method of purification of human erythropoietin involves immobilization of antibodies raised to erythropoietin on insoluble matrix, sorption of erythropoietin on immobilized antibodies and the following elution of erythropoietin. Monoclonal antibodies produced by cult...

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Hauptverfasser:	PIVOVAROV A.M, PROKOP'EV A.A, KOROBITSYN L.P, BELJAKOV N.V
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	FIELD: biotechnology. SUBSTANCE: method of purification of human erythropoietin involves immobilization of antibodies raised to erythropoietin on insoluble matrix, sorption of erythropoietin on immobilized antibodies and the following elution of erythropoietin. Monoclonal antibodies produced by cultured hybridoma clone taken among the group including clones PCE/6. PCE/10 and PCE/7 are used as antibodies. Erythropoietin is eluted with acid buffer solution, pH is less 4, and anion-exchange chromatography is used additionally for purification. Invention can be used for human erythropoietin preparing. EFFECT: enhanced effectiveness of process of human erythropoietin isolation. 5 cl, 6 dwg, 1 tbl, 7 ex