METHOD OF BONE MARROW PRESERVATION

FIELD: medicine. SUBSTANCE: method may be used in hematologic and oncologic centers for treatment of patients by means of transplantation of preserved bone marrow. Method includes step-by-step freezing in presence of cryoprotective solution, at which suspension of cells is filled by 10-30% in soft c...

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Bibliographische Detailangaben
1. Verfasser:	KOSTJAEV A.A
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES FISHING FORESTRY HUMAN NECESSITIES HUNTING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF TRAPPING
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Beschreibung
Zusammenfassung:	FIELD: medicine. SUBSTANCE: method may be used in hematologic and oncologic centers for treatment of patients by means of transplantation of preserved bone marrow. Method includes step-by-step freezing in presence of cryoprotective solution, at which suspension of cells is filled by 10-30% in soft container of one-time use. Container is placed in metal case which provides for thickness of suspension layer equal to 6-8 mm. Case with suspension of cells is placed at first step in liquid cooling agent at temperature of 26-30 C and kept in it for 15-25 min. At the second step case with suspension is arranged in storage-refrigerator at temperature of minus 40-50 C. The method ensures reliable safety of full morphological and functional value of depreserved myelocaryocytes and committed cells-predecessors after 120 days of bone marrow storage. EFFECT: higher efficiency of preservation. 2 ex