METHOD OF DETECTION OF AGRICULTURE ANIMALS INFECTED WITH CHLAMYDIA PSITTACI

FIELD: veterinary science, microbiology, molecular biology. SUBSTANCE: method is based on construction of recombinant plasmid pCP-III containing specific Bam HI-fragment of chromosomal DNA of Chlamydia psittaci. Recombinant plasmid can be used for detection of agriculture animals infected with chlam...

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Hauptverfasser: CHERNOV V.M, GOGOLEV JU.V, AVZALOV F.Z, IDRISOV G.Z, CHERNOVA O.A, GAFFAROV KH.Z
Format: Patent
Sprache:eng
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Zusammenfassung:FIELD: veterinary science, microbiology, molecular biology. SUBSTANCE: method is based on construction of recombinant plasmid pCP-III containing specific Bam HI-fragment of chromosomal DNA of Chlamydia psittaci. Recombinant plasmid can be used for detection of agriculture animals infected with chlamydia by method of DNA-DNA hybridization on nitrocellulose filters. The sensitivity of method is less 1 ng DNA of pathogen. Method can be used for diagnosis of chlamydiosis in veterinary medicine. EFFECT: improved method of detection. 3 dwg