CHROMATOGRAPHY METHOD OF ISOLATION AND PURIFICATION OF PROTEINS, PEPTIDES AND THEIR COMPLEXES

CHROMATOGRAPHY METHOD OF ISOLATION AND PURIFICATION OF PROTEINS, PEPTIDES AND THEIR COMPLEXES

FIELD: biochemistry, medicine, endocrinology. SUBSTANCE: human insulin obtained from preproinsulin where natural methionine located between leader sequence is substituted with arginine is isolated and purified by ion-exchange chromatography. Method ensures to obtain insulin without toxic reagents us...

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Hauptverfasser: MARKIN S.S, SEMENOV M.P, NECHAEV V.N, UVAROV V.JU, SERGIENKO V.I
Format: Patent
Sprache:eng
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CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
SEPARATION
TRANSPORTING
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creator MARKIN S.S
SEMENOV M.P
NECHAEV V.N
UVAROV V.JU
SERGIENKO V.I
description FIELD: biochemistry, medicine, endocrinology. SUBSTANCE: human insulin obtained from preproinsulin where natural methionine located between leader sequence is substituted with arginine is isolated and purified by ion-exchange chromatography. Method ensures to obtain insulin without toxic reagents using. EFFECT: simplified technology, high yield and purity of product. 4 cl, 1 ex
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subjects CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
SEPARATION
TRANSPORTING
title CHROMATOGRAPHY METHOD OF ISOLATION AND PURIFICATION OF PROTEINS, PEPTIDES AND THEIR COMPLEXES
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