CHROMATOGRAPHY METHOD OF ISOLATION AND PURIFICATION OF PROTEINS, PEPTIDES AND THEIR COMPLEXES

FIELD: biochemistry, medicine, endocrinology. SUBSTANCE: human insulin obtained from preproinsulin where natural methionine located between leader sequence is substituted with arginine is isolated and purified by ion-exchange chromatography. Method ensures to obtain insulin without toxic reagents us...

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Hauptverfasser:	MARKIN S.S, SEMENOV M.P, NECHAEV V.N, UVAROV V.JU, SERGIENKO V.I
Format:	Patent
Sprache:	eng
Schlagworte:	CHEMISTRY METALLURGY ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL SEPARATION TRANSPORTING
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Beschreibung
Zusammenfassung:	FIELD: biochemistry, medicine, endocrinology. SUBSTANCE: human insulin obtained from preproinsulin where natural methionine located between leader sequence is substituted with arginine is isolated and purified by ion-exchange chromatography. Method ensures to obtain insulin without toxic reagents using. EFFECT: simplified technology, high yield and purity of product. 4 cl, 1 ex