METHOD OF DETECTION OF PIG CLASSIC PLAGUE VIRUS

FIELD: molecular biology, genetic engineering. SUBSTANCE: method involves synthesis of oligonucleotide primers 5'-CTGCTGGCCTGG- -GTGACTACG-3' and 5'-ATAACTCAATGGAACCTG-3' and synthesis of complementary DNA on RNA-template. Then amplification of synthesized DNA is carried out by m...

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Hauptverfasser: ORESHKOVA S.F, PUZYREV A.T, DONCHENKO A.S, SEMENIKHIN V.I, CHEKISHEV V.M, IL'ICHEV A.A, GERB P.E
Format: Patent
Sprache:eng
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Zusammenfassung:FIELD: molecular biology, genetic engineering. SUBSTANCE: method involves synthesis of oligonucleotide primers 5'-CTGCTGGCCTGG- -GTGACTACG-3' and 5'-ATAACTCAATGGAACCTG-3' and synthesis of complementary DNA on RNA-template. Then amplification of synthesized DNA is carried out by method of polymerase chain reaction and size products of polymerase chain reaction is analyzed. Method ensures to differentiate pig classic plague virus from closely related cattle diarrhea virus. EFFECT: high specificity of method. 1 tbl, 1 ex