RECOMBINANT PLASMID DNA PGGF-8 ENCODING POLYPEPTIDE SHOWING PROPERTIES OF HUMAN GRANULICYTIC COLONY-STIMULATING FACTOR AND THE STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF POLYPEPTIDE SHOWING PROPERTIES OF HUMAN GRANULOCYTIC COLONY-STIMULATING FACTOR

FIELD: biotechnology, genetic engineering. SUBSTANCE: recombinant plasmid DNA has synthetic gene encoding human granulocytic colony-stimulating factor, promoter tandem of tryptophan operon of E.coli and synthetic enhancer of gene-10 translation of bacteriophage 17. Strain Escherichia coli as a produ...

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Hauptverfasser:	PUSTOSHILOVA N.M, PETRENKO L.A, KOROBKO V.G, PETROVSKAJA L.E, SHINGAROVA L.N
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	FIELD: biotechnology, genetic engineering. SUBSTANCE: recombinant plasmid DNA has synthetic gene encoding human granulocytic colony-stimulating factor, promoter tandem of tryptophan operon of E.coli and synthetic enhancer of gene-10 translation of bacteriophage 17. Strain Escherichia coli as a producer of this polypeptide is obtained. Biosynthesis of polypeptide shows constitutive character and its level is 10% of total cellular protein content, not less. Invention can be used for preparing human granulocytic colony-stimulating factor. EFFECT: recombinant plasmid DNA and strain of bacterium indicated above, increased production of factor. 2 cl, 4 dwge