METHOD OF PREPARING ANTIGEN FOR GLANDERS DIAGNOSIS IN AGGLUTINATION REACTION

FIELD: veterinary immunology. SUBSTANCE: antigen is prepared by glanders pathogen culturing on nutrient medium from Kursk biofactory for 10-14 days. Grown culture is concentrated and purified by diafiltration on membranes (pore size is 0.1-0.22 mcm). Then cells are stained with 1% solution of an org...

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Hauptverfasser: SUKHANOV V.P, SHAROV A.N, SHEVYREV N.S, KOZLOV V.E, KLIMANOV A.I, KOROVENKOV A.I, NICHVEEVA L.D, BUKOVA N.K, GRECHKINA N.T, BEZGIN V.M
Format: Patent
Sprache:eng
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Zusammenfassung:FIELD: veterinary immunology. SUBSTANCE: antigen is prepared by glanders pathogen culturing on nutrient medium from Kursk biofactory for 10-14 days. Grown culture is concentrated and purified by diafiltration on membranes (pore size is 0.1-0.22 mcm). Then cells are stained with 1% solution of an organic dye amount of that is assayed by titration. Obtained antigen can be used in veterinary science for express-diagnosis of glanders by detection of antiglanders antibodies in blood serum of infected animals. EFFECT: improved method of preparing, enhanced effectiveness of diagnosis. 2 cl, 1 tbln