METHOD OF HYDROLYZATE PREPARING AND THEIR USING FOR NUTRIENT MEDIA PREPARING FOR MICROORGANISM CULTURING
FIELD: microbiology. SUBSTANCE: to release amino acids from protein-containing waste the latter is subjected for contact with 26% ammonia aqueous solution and hydrolyzable substrate is milled. Raw is diluted preliminary with water at ratio 1:1.5, 1:5 and 1:1.6 for globulins, chicken embryos and fibr...
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| creator | KOLPAKOVA RAISA GEORGIEVNA SIT'KOV VIKTOR IVANOVICH TUTOV IVAN KIRILLOVICH SURMILO ALEKSEJ PETROVICH ZAERKO VIKTOR IVANOVICH |
| description | FIELD: microbiology. SUBSTANCE: to release amino acids from protein-containing waste the latter is subjected for contact with 26% ammonia aqueous solution and hydrolyzable substrate is milled. Raw is diluted preliminary with water at ratio 1:1.5, 1:5 and 1:1.6 for globulins, chicken embryos and fibrin and blood clots, respectively. To prepared raw 26% ammonia aqueous solution is added as measured 19 ml/1 l mixture and water. Hydrolysis is carried out at 120 C for 2 h, hydrolyzate is cooled to 45 C and pH value is brought about to 7.0 with hydrochloric acid solution. Then material is hydrolyzed with enzyme from cattle pancreas. Enzymatic hydrolysis is carried out at 42-43 C for 6-7, 6 and 4-5 days for globulins, chicken embryos and fibrin and blood clots, respectively. Method can be used for industrial production of bacterial vaccines. EFFECT: improved method of preparing. 4 cl, 2 tblf |
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SUBSTANCE: to release amino acids from protein-containing waste the latter is subjected for contact with 26% ammonia aqueous solution and hydrolyzable substrate is milled. Raw is diluted preliminary with water at ratio 1:1.5, 1:5 and 1:1.6 for globulins, chicken embryos and fibrin and blood clots, respectively. To prepared raw 26% ammonia aqueous solution is added as measured 19 ml/1 l mixture and water. Hydrolysis is carried out at 120 C for 2 h, hydrolyzate is cooled to 45 C and pH value is brought about to 7.0 with hydrochloric acid solution. Then material is hydrolyzed with enzyme from cattle pancreas. Enzymatic hydrolysis is carried out at 42-43 C for 6-7, 6 and 4-5 days for globulins, chicken embryos and fibrin and blood clots, respectively. Method can be used for industrial production of bacterial vaccines. 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SUBSTANCE: to release amino acids from protein-containing waste the latter is subjected for contact with 26% ammonia aqueous solution and hydrolyzable substrate is milled. Raw is diluted preliminary with water at ratio 1:1.5, 1:5 and 1:1.6 for globulins, chicken embryos and fibrin and blood clots, respectively. To prepared raw 26% ammonia aqueous solution is added as measured 19 ml/1 l mixture and water. Hydrolysis is carried out at 120 C for 2 h, hydrolyzate is cooled to 45 C and pH value is brought about to 7.0 with hydrochloric acid solution. Then material is hydrolyzed with enzyme from cattle pancreas. Enzymatic hydrolysis is carried out at 42-43 C for 6-7, 6 and 4-5 days for globulins, chicken embryos and fibrin and blood clots, respectively. Method can be used for industrial production of bacterial vaccines. EFFECT: improved method of preparing. 4 cl, 2 tblf</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD OF HYDROLYZATE PREPARING AND THEIR USING FOR NUTRIENT MEDIA PREPARING FOR MICROORGANISM CULTURING |
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