COMPOSITION AND PROCESS FOR PREPARING HYDROGELS BASED ON SALECAN AND FOR USING THE SAME FOR ADDITIVE MANUFACTURING

The invention relates to a process for preparing hydrogels to be used for manufacturing 3D-printed cross-linked structures. According to the invention, the process comprises the following steps: preparing solutions of 5...15% citric acid in ultrapure water, hydrating 1...10% salecan polysaccharide in the citric acid solution while mechanically stirring at 600...800 rpm and at a temperature of 25...40°C for 4...6 h, increasing the temperature and maintaining the hydrogel compositions at 50°C for 10...20h, homogenizing by means of an ultra-homogenization probe for 2...5 min, centrifuging for 10...20 min at 7000...9000 rpm, moulding in a Teflon mould, drying by freeze drying and thermally treating the xerogels at a temperature of 40...60°C for 20...30 h and at 100...150°C for 5...30 min, resulting in cross-linked hydrogels with spongy structures with interconnected pores that allow the encapsulation of bioactive agents, as well as cell adhesion and proliferation. Invenţia se referă la un procedeu de obţinere a unor hidrogeluri utilizate pentru fabricarea unor structuri reticulate printate 3D. Procedeul, conform invenţiei, constă în etapele: preparare a unor soluţii de 5...15% acid citric în apă ultrapură, hidratare a 1...10% polizaharidă salecan în soluţia de acid citric sub agitare mecanică de 600...800 rpm şi temperatura de 25...40°C timp de 4...6h, creşterea temperaturii şi menţinerea compoziţiilor de hidrogel la 50°C timp de 10...20 h, omogenizarea cu ajutorul unei sonde de ultraomogenizare timp de 2...5 min, centrifugare timp de 10...20 min la 7000...9000 rpm, formarea în matriţă de teflon, uscare prin liofilizare şi tratament termic al xerogelurilor la temperatura de 40...60°C timp de 20...30 h şi la 100...150°C timp de 5...30 min, rezultând hidrogeluri reticulate cu structuri spongioase cu pori interconectaţi care permit încapsularea de agenţi bioactivi, precum şi adeziunea şi proliferarea celulară.