ELECTROCHEMICAL BIOSENSOR FOR DETERMINING URIC ACID IN BIOLOGICAL FLUIDS

The invention relates to a process for making an electrochemical biosensor for detecting uric acid in biological fluids. According to the invention, the process consists of the following stages: manufacturing an electrode of electrospun polymer fibers of micro- and nano-metric sizes coated with pall...

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Hauptverfasser: ENACHE TEODOR ADRIAN, BRANCO LEOTE RICARDO JOSE, IGNAT BARSAN MĂDĂLINA MARIA, ENCULESCU MARIA-MONICA, DICULESCU VICTOR CONSTANTIN
Format: Patent
Sprache:eng ; rum
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Zusammenfassung:The invention relates to a process for making an electrochemical biosensor for detecting uric acid in biological fluids. According to the invention, the process consists of the following stages: manufacturing an electrode of electrospun polymer fibers of micro- and nano-metric sizes coated with palladium, immobilizing uricase on the electrode surface, measuring the current of reducing the superficial PdO layer formed at the surface of the electrode by reaction with H2O2, with uric acid detection limits lower than the usual methods of analysis. Invenţia se referă la un procedeu de obţinere a unui biosenzor electrochimic pentru detecţia acidului uric în fluide biologice. Procedeul, conform invenţiei, constă în etapele de: realizare a unui electrod din fibre polimerice electrofilate cu dimensiuni micro- şi nano-metrice acoperite cu paladiu, imobilizarea de uricază pe suprafaţa electrodului, măsurarea curentului de reducere a stratului superficial de PdO format la suprafaţa electrodului prin reacţia cu H2O2, cu limite de detecţie a acidului uric inferioare metodelor uzuale de analiză.